Problem:

Question 1: What would be the consequence of treating the vector, before ligation, with calf intestinal phosphatase?

A. It would prevent the plasmid's DNA from reversing polarity during ligation.

B. It would prevent the ends of the plasmids from being ligated.

C. It would ligate the ends of the plasmids.

D. It would reverse the polarity of plasmids' DNA

Question 2: What is generally the starting material for whole genome shotgun sequencing of genomic DNA?

A. Transposon-tagged DNA fragments

B. Overlapping fragments of genomic DNA

C. Genomic DNA fragments flanked by repetitive DNA

D. A collection of cDNAs from a particular cell type

Give reasoning with your answer.