Problem:

Please describe the answer of the following questions:

Question 1: Identify the steps taken in the preparation of a bacterial smear to prevent contamination of the culture and yourself working with a bacterial smear?

Question 2: Summarize the "fundamental theory" of simple staining. hint: what scientific property allows the dyes t bind the bacterial cell wall?

Question 3: What would happen if you placed a large drop of water on a slide rather than a loopful, when preparing a bacterial smear?

Question 4: Discuss the possible outcomes if an air-dried smear were not heat fixed before proceeding to the staining step.

Question 5: While viewing a slide of a "pure" culture you observe two different shaped bacteria; some rod shaped while others are spherical. How could this happen? List at least two explanations and/or sources for this result.

Please explain all the answers in detail.