Problemon a given microscope the numerical apertures of the, Ask an Expert

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Problem:

On a given microscope, the numerical apertures of the condenser and low power objective lenses are 1.25 and 0.25, respectively. You are supplied with a filter that selects a wavelength of 520nm.

Question 1: What is the limit of resolution on this microscope? Please be specific to how you came up with this answer, what formula did you use?

Question 2: Will you be able to distinguish 2 points that are 300nm apart as being separate, or will they blur into one?

The high dry objective lens has a numerical aperture of 0.85, what is the limit of resolution on this microscope, and will you be able to distinguish 2 points that are 300 nm apart as being separate, or will they blur into one? Explain answer.

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Category:- Biology
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