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Problem:

Labeled pyruvate containing 14C ( 200 mCi/gm at ) C-3 ( the methyl carbon), 13 C( 90% enriched) at C-2 and 18 O ( 80%enriched) at C-2m was added to a pigeon breast muscle preparation, in order to investigate the citric acid cycle.

Required:

Question: How man turns of TCA cycle are required for all the radioactivity ( 14C to be depleted)??


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