Problemive used hplc high performance liquid chromatography, Ask an Expert

Academics

Problem:
I've used HPLC (high performance liquid chromatography) before (once, so I'm barely even qualified to know what it stands for) so I was surprised when my lab mate told me she would be using an alternate technique to isolate a protein.
Question: What exactly are differences in HPLC and FPLC (fast protein liquid chromatography) instruments?
Question: Why would FPLC be a better technique than HPLC to use with proteins?
Question: What situations make HPLC preferable?
Please explain the difference , provide better techniques and define the situations that make HPLC preferable.

View complete question

Biology, Academics

Category:- Biology
Reference No.:- M91146225

Have any Question?Write your Review or question?

Related Questions in Biology

Is the iso-osmotic point different for different solutes

Is the iso-osmotic point different for different solutes? Please specify in what ways can the iso-osmotic points be different and why there is a difference.

In summer squash white fruit w is dominant over yellow

In summer squash, white fruit (W) is dominant over yellow fruit (w) and the "disk" fruit shape (D) is dominant over "sphere" shape (d). Determine the genotypes of the parents in the following cases: A. White disk crossed ...

1 identify the maternal homolog for both chromosome 1 and 2

1.) Identify the maternal "homolog" for both Chromosome 1 and 2 and then the paternal homologs. Arrange these chromosomes as you would expect to find them during G 1 of interphase. What is the haploid number of our ce ...

Discussion bad blood a case study of the tuskegee syphilis

Discussion Bad Blood: A Case Study of the Tuskegee Syphilis Project. Photo by Department of Health, Education, and Welfare. Public Health Service. Health Services and Mental Health Administration. Center for Disease Cont ...

Bioinformatics assignment -in this assignment should check

Bioinformatics Assignment - In this assignment should check the following sequence and test whether it has the following restriction cut sites. This searching should be done globally, that is, it should check for all pos ...

Question dietary recommendation and analysis projectin this

Question: Dietary recommendation and analysis project In this project, you will use software to analyze our own diet and make dietary recommendation. You will need submit a report. You can choose any of software if you f ...

Why biologically is evolved resistance to crop pests

Why, biologically, is evolved resistance to crop pests inevitable?

What are the equations for molarity molality density ppm

What are the equations for Molarity, molality, density, ppm, ppb, mass percent, Volume percent, and mole percent?

Why soap changes the surface tension of waterand why adding

Why soap changes the surface tension of water? And why adding water into penny, the average of water drops in 90oC temperature water is less than average of water drops in room temperature

Soap and detergent molecules have a long hydrophobic tail

Soap and detergent molecules have a long, hydrophobic tail and a polar, hydrophilic head. They are sometimes referred to as bridge molecules because they allow oils and fats to be suspended and dissolved in water (whic ...

4,153,160 Questions Asked
13,132 Experts
2,558,936 Questions Answered

Ask Experts for help!!

Looking for Assignment Help?

Start excelling in your Courses, Get help with Assignment

Write us your full requirement for evaluation and you will receive response within 20 minutes turnaround time.

Ask Now Help with Problems, Get a Best Answer

Recent Questions

Ask Biology Expert