Problem:

Isolation of Chloroplasts

Question 1: Do all the procedures quickly to minimize keeping the materials outside too long Keep materials ice cold as much as possible

Question 2: Why do we need to keep all the solutions ice-cold?

Question 3: Why 60%, 50%, 40%, and 30% sucrose gradient centrifuge tube was prepared?

Question 4: In assay of chlorophyll content using a spectrophotometer at 652 nm, why at 652 nm?

Question 5: Why Acetone was added to the chlorophyll solution?

Question 6: Why distilled water is added to the solution?

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