Probleminstead of using polymerase vent exo - can i go with, Ask an Expert

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Instead of using polymerase Vent exo (-), can I go with the usual Taq polymerase?

Question: Do the PCR conditions change (the temperature and master mix concentrations ) in the these two conditions?

Question: Do the primers (sequence and concentrations) change if we use vent exo instead of Taq polymerase and vice versa?

Question: If the primers are designed specifically for Vent exo (-) how can I modify the PCR conditions so that I can use the same primers (meant for vent exo) using Taq polymerase? Justify your answer.

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Biology, Academics

Category:- Biology
Reference No.:- M91148989

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