Problemin the production of recombinant proteinsquestion 1, Ask an Expert

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Problem:

In the production of recombinant proteins:

Question 1: What are the essential features of any affinity tag that has been developed for use in purification of proteins?

Question 2: What added benefits can affinity tags give to improve the properties or yield of a recombinant proteins?

Question 3: You are given a test tube with 1 ml of a recombinant protein that your colleague has just purified in the lab using affinity purification. How would you attempt to determine the concentration and purity of that protein?

Give detailed discription of all the answers.

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Biology, Academics

Category:- Biology
Reference No.:- M91149769

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