Problemin pretty much every transformation protocol ive, Ask an Expert

Academics

Problem:
In pretty much every transformation protocol I've seen SOC medium is used to grow the bacteria for a short while after the transformation and before plating.
I've usually substituted LB medium for this step for easy retransformations, but I gather that the SOC medium is supposed to make some difference in efficiency, or else the simpler LB medium would be recommended in the protocols.
Question: Why is SOC medium used for transformations?
Question: What difference does substituting with LB medium cause for the transformation efficiency?
Provide better results for the following question.

View complete question

Biology, Academics

Category:- Biology
Reference No.:- M91146233

Have any Question?Write your Review or question?

Related Questions in Biology

How do specimens move when put under the dissecting

How do specimens move when put under the dissecting microscope?

Use of molecular tools and bioinforrnatics in the diagnosis

Use of Molecular Tools and Bioinforrnatics in the Diagnosis Characterization of Enteric Pathogens from a Case Study Purpose: The purpose of this project is to familiarize the student with modern molecular tools and bioin ...

Why soap changes the surface tension of waterand why adding

Why soap changes the surface tension of water? And why adding water into penny, the average of water drops in 90oC temperature water is less than average of water drops in room temperature

Question darwin was not the first to consider evolution as

Question: Darwin was not the first to consider evolution as a process but he did come up with the first effective explanation for how it happens. Describe Darwin's theory of evolution by natural selection. Explain how th ...

What are the equations for molarity molality density ppm

What are the equations for Molarity, molality, density, ppm, ppb, mass percent, Volume percent, and mole percent?

Question identify an organism that lives within 50 miles of

Question: Identify an organism that lives within 50 miles of your home. Write a 1,050- to 1,400-word paper about how the organism has adapted to survive in their specific environment. Include the following points in your ...

A how are chromosomes positioned on the metaphase

a. How are chromosomes positioned on the metaphase plate in metaphase 1 of meiosis? b. Is this different from metaphase in mitosis? If so, how?

Question 1go outside during daylight hours to a natural or

Question 1. Go outside during daylight hours to a natural or semi-natural space (e.g., Burton's Pond, an urban park, your backyard). Sit still for 20 minutes and closely observe the plants and animals around you. Describ ...

What is the complementary strand for 5-atgcatgcatgccc-3how

What is the complementary strand for: 5'-ATGCATGCATGCCC-3' How many turn(s) will this strand have? Are Eukaryotic cells always diploid during S phase whereas bacteria are only haploid at the end of DNA replication?

Which are the logical steps proposed by margulis that would

Which are the logical steps proposed by Margulis that would help explain why the accumulation of oxygen in the atmosphere led to the evolution of mitochondria.

4,153,160 Questions Asked
13,132 Experts
2,558,936 Questions Answered

Ask Experts for help!!

Looking for Assignment Help?

Start excelling in your Courses, Get help with Assignment

Write us your full requirement for evaluation and you will receive response within 20 minutes turnaround time.

Ask Now Help with Problems, Get a Best Answer

Recent Questions

Ask Biology Expert