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Problem:

I work with mice, and I want to see what happens to some specific proteins in the mouse brain after IL-1b injection (intracerebroventricular).
I have a problem: when I measure the mRNA and protein levels, I see that there are some differences between them.
It can be like: mRNA (upregulated) Protein (no difference)

Required:

Question 1: Is it possible that these proteins have a high turnover rate? If so, how can I prevent that?

Question 2: Can I (if it is possible) inject (intracerebroventricular) IL-1b mixed with protease inhibitors?
Please answer the questions.

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Biology, Academics

Category:- Biology
Reference No.:- M91149021

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