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Problem:

I just wanted to understand the basic steps behind the replication of the lagging strand of DNA:

Have helicase unwind it first

  • DNA Primase lays down RNA primers in fragments, called Okazaki fragments
  • DNA polymerase III goes through and corrects all the mistakes (essentially replace the Uracil with Thymine)
  • DNA polymerase I goes through and removes RNA primer
  • DNA ligase hooks up each of the 5' to 3' end fragments

Required:

Question: Is this the correct order of steps?

Question: Does the leading strand need any RNA primer or does the DNA polymerase just start and go to the end without any help?

Biology, Academics

  • Category:- Biology
  • Reference No.:- M91162813

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