Problemi have a validated set of primers for rtpcr from, Ask an Expert

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Problem:

I have a validated set of primers for rtPCR from Biorad that contains SYBR green.

Question: If I do rtPCR, can I use the rtPCR product after purifying it with a Qiagen PCR purification kit? Please justify your answer.

Also, I'm under the impression that the sequencing company needs primers to do sequencing,

Question: Do I just use the PCR primer or one of the "universal" primers the company has?

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Biology, Academics

Category:- Biology
Reference No.:- M91149376

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