Problemi am performing a gel extraction to purify dna after, Ask an Expert

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Problem:

I am performing a gel extraction to purify DNA after a double digest with EcoRI and BamHI. After the gel extraction I need to complete a ligation step before bacterial transformation. The problem I am having is that the DNA after the gel extraction have some kind of chemical contaminant that won't allow me to quantify the DNA using the Nanodrop spectrophotometer. I am getting very low 260/230 ratios.

Question: Has anyone had some issues with this?

I am using the Qiagen Qiaquick Gel Extraction Kit.

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Biology, Academics

Category:- Biology
Reference No.:- M91149227

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