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Has anyone optimized RT for long transcripts (9kb)? The downstream application will be PCR amplification and Illumina library prep. It will be trivial to make internal primers sets for the PCR that are specific as long as there are no chimeric sequences. If there are, they will probably get primed also. If anyone knows of an optimization and/or other potential pitfalls, I would love to hear them.

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Category:- Biology
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