Problem:

Further research shows that the purified happyase* used in the first two experiments was actually contaminated with a reversible inhibitor called ANGER.

Required:

Question: When ANGER is carefully removed from the happyase* preparation and the two experiments repeated, the measured V max in

(a) is increased to 4.8 ? M s ? 1 , and the measured K m in

(b) is now 15 ? M . For the inhibitor ANGER, calculate the values of ? and ? 9

Show the pedigree and your calculation.