Problemduring electroporation of bacterial cells i work, Ask an Expert

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Problem:
During electroporation of bacterial cells (I work with Mycobacterium tuberculosis, but I think this applies to E. coli as well), sometimes I get sparking. I've read this is due to salts present, either in the DNA sample or the bacteria.

I use the Qiagen plasmid prep kit, which includes EB (Elution Buffer). This contains Tris-Cl.

Question 1: Could this be contributing to the sparking?

Question 2: Can you explain how this works?

Question 3: Does eluting with water instead of EB reduce chances of sparking?

Explain all the answers step by step.

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Biology, Academics

Category:- Biology
Reference No.:- M91147040

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