Ask Question, Ask an Expert

+61-413 786 465

info@mywordsolution.com

Ask Biology Expert

Problem:

During DNA isolation, you incubated the tissue sample at 55°C for 60 minutes, followed by an incubation at 95°C for 10 minutes.

Required:

Question: Why was this last 95°C incubation necessary?

Question: What would happen to the PCR if this step was omitted?


Does anyone know how to go about this search to explain it.

Biology, Academics

  • Category:- Biology
  • Reference No.:- M91163299

Have any Question?


Related Questions in Biology

A cross between a person with straight hair and a person

A cross between a person with straight hair and a person with curly hair will produce a child with wavy hair. If 2 people with wavy hair had a child, what are the odds that the child would have straight hair?

Experiment 1 symmetry in common objectsreview the objects

Experiment 1: Symmetry in Common Objects Review the objects listed below (many of which can be found in your lab kit). Decide what type of symmetry they possess. Explain why you chose the type of symmetry you did. 1. Saf ...

Is this statement correct or incorrect whymuscle tension in

Is this statement correct or incorrect? Why? Muscle tension in cardiac contractile muscle can be increased by temporal summation.

Assignment 2 biological basiscontinuing on the research

Assignment 2: Biological Basis Continuing on the research that you started in Week 3, explain what your chosen biotechnology accomplishes and how it is implemented, and describe the body of knowledge that it is based upo ...

A mother has a blood the child has type o mr white has type

A mother has A blood, the child has type O, Mr. White has type B blood, and Mr. Green has AB. Whose the father? Mr. White or Mr. Green; Either Mr. White or Mr. Green; Neither Mr. White or Mr. Green could be the father an ...

Question this is the final project of the case

Question: This is the final project of the case study 1. Identify the specific case study you chose and the tool(s) you would employ to analyze the healthcare environment. 2. Provide an explanation of how the selected to ...

The human microbiome and its relationship to human health

The human microbiome and its relationship to human health, what aspect to the human microbiome is vital?

Oxidative decarboxylationwhat is itwhat are the

Oxidative decarboxylation What is it? What are the reactants? What are the products? What is the net ATP gain? Where does it take place in prokaryotes vs eukaryotes?

In summer squash white fruit w is dominant over yellow

In summer squash, white fruit (W) is dominant over yellow fruit (w) and the "disk" fruit shape (D) is dominant over "sphere" shape (d). Determine the genotypes of the parents in the following cases: A. White disk crossed ...

Question - gene cloning a please write the steps to clone

Question - Gene Cloning a) Please write the steps to clone the protease gene from Bacillus strain whose genome sequence is not known. b) Express the protease gene to obtain the enzyme in high yield, please plan your prot ...

  • 4,153,160 Questions Asked
  • 13,132 Experts
  • 2,558,936 Questions Answered

Ask Experts for help!!

Looking for Assignment Help?

Start excelling in your Courses, Get help with Assignment

Write us your full requirement for evaluation and you will receive response within 20 minutes turnaround time.

Ask Now Help with Problems, Get a Best Answer

Why might a bank avoid the use of interest rate swaps even

Why might a bank avoid the use of interest rate swaps, even when the institution is exposed to significant interest rate

Describe the difference between zero coupon bonds and

Describe the difference between zero coupon bonds and coupon bonds. Under what conditions will a coupon bond sell at a p

Compute the present value of an annuity of 880 per year

Compute the present value of an annuity of $ 880 per year for 16 years, given a discount rate of 6 percent per annum. As

Compute the present value of an 1150 payment made in ten

Compute the present value of an $1,150 payment made in ten years when the discount rate is 12 percent. (Do not round int

Compute the present value of an annuity of 699 per year

Compute the present value of an annuity of $ 699 per year for 19 years, given a discount rate of 6 percent per annum. As