Problemduring dna isolation you incubated the tissue sample, Ask an Expert

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Problem:

During DNA isolation, you incubated the tissue sample at 55°C for 60 minutes, followed by an incubation at 95°C for 10 minutes.

Required:

Question: Why was this last 95°C incubation necessary?

Question: What would happen to the PCR if this step was omitted?

Does anyone know how to go about this search to explain it.

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Biology, Academics

Category:- Biology
Reference No.:- M91163299

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