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Question 1: What specific genetic manipulation does Synechocystis readily allow that E. coli does not?

Question 2: For transformation of Synechocystis, why do you first plate your transformant mixture on a filter on a BG - 11 plate with glucose, and the next day the TA transfers the filter with cells to a plate that contains kanamycin (rather than plating directly on kanamycin)?

Question 3: What is the product of the psbC gene? What is its function?

Question 4: In the PCR reaction, what is the role of each of the PCR reagents: primers, dNTPs, Mg2+, and Taq polymerase?

Question 5: What are the considerations in determining the number of cycles needed for the PCR reaction?

Please explain all the answers in detail.

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Category:- Biology
Reference No.:- M91148462

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