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Point at which proteins stop moving on a gel when they get a netcharge of zero.

pI = pKa(+1 charge) + pKa(-1 Charge)

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2-Average the pK values from +1 to -1 charge.

-Proteins Acidic if pI < 6

-Proteins Neutral if pI between: 6 - 8

-Proteins Basic if pI > 8

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