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- find out as many points on the curve as needed to discern its basic charcteristics.
- state any assumptions you have made
- plot points on a graph (simple excel graph is perfect): be sure calulcated point are visible with the curve (especially at inflection and equilibrium of the sigmoid, etc)
- general commens on what is going on in the curve

I hope this is now a little more straight forward. Your help would be greatly appreciated on this.

Here is the Problem:

(A) Assume you have a solution containing 0.1 mol of Ala adjusted to pH = 0.5 with HCl. You begin adding 1.0 M NaOH. Plot (do not sketch) the resulting titration curve (pH vs. moles NaOH added) showing all inflection points. Show your calculations and be sure to state your assumptions.

(B) Plot a similar titration curve for a solution containing 0.1 mol of His at pH = 0.5. (pKa values: Ala: alpha-COOH = 2.3, alpha-NH2 = 9.7; His: alpha-COOH = 1.8, alpha-NH2 = 9.2, Imidazole = 6.0).

• Category:- Biology
• Reference No.:- M924843

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