Question: Plasmid size of pGLO is 5371 bp and the restriction sites for PstI are 2106 and 3181. Compute to predict the sizes of fragments when gPLO is digested with PstI. Please show work.
Question: A microbiologist wants to make bacteria produce a foreign protein. He has cloned the protein gene into a plasmid. Which is the next step?
A. The plasmid is inserted into the main bacterial chromosome.