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Q1: What would be the effects of deleting the N-terminal signal series of the PDGF receptor in an epithelial cell?  Please describe both the effects on protein localization and on the cell’s capability to regulate its cell cycle.

Q2. A cell has a temperature-sensitive mutation in Ras GAP.  At low temperature the phenotype of the cell is normal. What phenotype would you expect to observe at the higher temperature?

Q3. Phosphotidyl inositol is made in the lumen of smooth endoplasmic reticulum.  How might a serious mutation in the flippase which generally distributes phosphotidyl inositol disrupt signaling via G-protein coupled receptors?

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