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Oxidation

Some enzymes which have the sulfhydryl (-SH) group in the catalytic site are very sensitive to oxidation. Due to oxidation of the -SH group by aerial oxygen or oxidizing agents, a disulfide linkage (-S-S-) forms with the subsequent loss of enzyme activity. The enzyme activity can be restored by the reduction of the enzyme by some reducing agent such as cysteine or glutathione.

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