Ask Question, Ask an Expert

+1-415-315-9853

info@mywordsolution.com

Ask Biology Expert

Let's assume that I fould a valuable gene fragment (ex. DNA sequence homology with one of the human myosin II genes)

1. Before I proceed for therapeutic development, How would I know if this gene is actually expressed in humans? What method?

2. Now let's assume it is expressed in human. I wish to express the cDNA corresponding to this gene in order to determine its function. How would you construct an expression vector? Would you use the genomic fragment or a cDNA?

3. How would you transfect your expression plasmid into cells, and what type(s) of cell might you use?

4. How would you confirm expression of your construct and encoded protein? (functions and methods)

Biology, Academics

  • Category:- Biology
  • Reference No.:- M927972

Have any Question? 


Related Questions in Biology

1 nbspnbsp is the fundamental structural andnbsp

1. __________   is the fundamental structural and  __________      unit of all living organisms a. cell, functional b. cell, microscopic c. nucleus, functional d. DNA, microscopic 2. Match the scientists with their respe ...

In the first stage of secondary succession grasses and

In the first stage of secondary succession, grasses and wildflowers abound. Why do these plants flourish where there used to be a forest?

Assignment lasa organisms in your biomein this assignment

Assignment: LASA: Organisms in Your Biome In this assignment, you will create a Microsoft PowerPoint presentation that exhibits the different organisms in your current biome. Include the following in your presentation: • ...

Case explain the activation and function of b cells in

Case : Explain the activation and function of B cells in body defences. What are the mechanisms of antibody actions which support immunity? How does HIV infection impair the body's ability to produce antibodies? Assignme ...

Effect of catalase on hydrogen Effect of Catalase on Hydrogen Peroxide Introduction:

Effect of Catalase on Hydrogen Peroxide Introduction: Metabolism is the sum total of chemical reactions in the body that are necessary to the maintenance of life. Enzymes are biological catalysts that can speed up, and c ...

Assignment anatomy amp physiologydirections be sure to

ASSIGNMENT: Anatomy & Physiology Directions: Be sure to save an electronic copy of your answer before submitting it to Ashworth College for grading. Unless otherwise stated, answer in complete sentences, and be sure to u ...

Make gene prediction of the provided sequenceusing both ab

Make gene prediction of the provided sequenceusing both ab initio (e.g. Z-curve) and homologybased approaches, and compare the predictionresults. Justify why the gene prediction programyou used is an appropriate one. Ins ...

Please answer the following questions you may answer on

Please answer the following questions, you may answer on this sheet or your own paper. 1. How does a theory differ from a hypothesis? 2. Mendel's laws are illustrated using Punnett squares. In the example below, we are l ...

What makes a dna fingerprint uniquei need to experiment and

What makes a DNA Fingerprint Unique? I need to experiment and create a lab report. Writing Lab Reports What lab reports and scientific papers do: - Persuade others to accept or reject hypotheses by presenting data and in ...

Researchers are working to develop biofuels to free us from

Researchers are working to develop biofuels to free us from dependence on fossil fuels. Based on what you know about cellulose, what do you predict are the major advantage and disadvantage of using cellulose-rich plant m ...

  • 4,153,160 Questions Asked
  • 13,132 Experts
  • 2,558,936 Questions Answered

Ask Experts for help!!

Looking for Assignment Help?

Start excelling in your Courses, Get help with Assignment

Write us your full requirement for evaluation and you will receive response within 20 minutes turnaround time.

Ask Now Help with Problems, Get a Best Answer

A cola-dispensing machine is set to dispense 9 ounces of

A cola-dispensing machine is set to dispense 9 ounces of cola per cup, with a standard deviation of 1.0 ounce. The manuf

What is marketingbullwhat is marketing think back to your

What is Marketing? • "What is marketing"? Think back to your impressions before you started this class versus how you

Question -your client david smith runs a small it

QUESTION - Your client, David Smith runs a small IT consulting business specialising in computer software and techno

Inspection of a random sample of 22 aircraft showed that 15

Inspection of a random sample of 22 aircraft showed that 15 needed repairs to fix a wiring problem that might compromise

Effective hrmquestionhow can an effective hrm system help

Effective HRM Question How can an effective HRM system help facilitate the achievement of an organization's strate