You characterize a new enzyme's kinetics.
You characterize a new enzyme's kinetics. Using a saturating (very high so all E is ES) substrate concentration, you measure the initial rate of the reaction at various enzyme concentrations. Does this data fit the Michaelis Menton model/equation? If not, what may be happening?
E (nM), rate (mM,s)
A. NO, the rate should only vary with substrate concentration, not enzyme concentration. The person collecting the data doesn't have a clue.
B. No, the activity does not change with increasing enzyme concentration correctly. The enzyme may be only active in a dimer that forms a high concentration.
C. Yes, it obeys the MM equation well.
D. No, the activity does not change with increasing enzyme concentration correctly. The enzyme may be inactive in a dimer that forms at high concentration
E. No, the rate should decrease as you increase the enzyme concentration. This reaction is not enzyme catalyzed.