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Niremberg used the enzyme polynucleotide phosphorylase to assembleRNAs with random sequences in the absence of a template. Supposethey added the enzyme to a solution of 90%CTP and 10%UTP, and thenused a cell-free system of protein synthesis to make peptidesaccording to the sequences in their synthetic RNAs.

a.) Which amino acids could possibly appear in the syntheticprotein?
b.) Which would be most abundant?
c.) Which would be least abundant?

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