Multiple choice  SOLUTIONS

1. The difference in RNA bases compared to DNA bases is

A) RNA contains A instead of T. D) RNA contains A instead of U.
B) RNA contains U instead of G. E) None of the above.
C) RNA contains U instead of T.

2. What is the nucleotide sequence on the DNA template strand that yields the CUA codon for leucine?
A) AUC D) GAT
B) TAG E) CUA
C) CTA

3. What is the nucleotide sequence on the coding DNA strand that yields the CUA codon for leucine?
A) AUC D) GAT
B) TAG E) GUA
C) CTA

4. What are the common promoter regions found in bacterial genes?
A) a Pribnow box about -10 and a -35 region
B) a TATA box about -25 and a CAAT box about -75
C) a Pribnow box about -10 and a TATA box about -25
D) a TATA box about -25 and a -35 region
E) None of the above.

5. Replication moves from
A) 5' → 3' direction. D) 3' → 3' direction.
B) 3' → 5' direction. E) none of the above.
C) 5' → 5' direction.
.
6. Functions of RNA polymerase include
A) searching for promoter sites. D) a and c.
B) unwinding short stretches of DNA. E) a, b, and c.
C) detecting termination signals.

7. Which of the following components of the tRNA is (are) important for the binding specificity of the tRNA synthetase?
A) acceptor stem D) a and b
B) anticodon loop E) a, b, and c
C) TΨC loop

8. Which of the following is (are) required for initiation of protein synthesis in prokaryotes?
A) mRNA
B) the 30S subunit
C) fmet-tRNAf
D) GTP
E) All of the above.

9. What is the order of the tRNA binding sites on the 70S ribosome with respect to the direction of the mRNA?
A) E P A
B) A P E
C) A E P
D) E A P
E) E A P

10. Signals that define the beginning and end of protein synthesis are contained in

A) rRNA. B) tRNA. C) mRNA. D) ribosome. E) protein.

11. Eukaryotic and prokaryotic translation differ primarily in the ________ step.
A) initiation D) all of the above
B) elongation E) none of the above
C) termination

Consider the replicating fork to the right.  It is part of a replicating “bubble”, part of a chromosome being replicated.

12. Which piece is the template for the leading strand?
a.1 to 3'b. 6 to 7 c. 2 to 3 d. 5 to 4
e. None of these are correct.

13. Which piece is being synthesized discontinuously?

a.1 to 3'b. 6 to 7 c. 3 to 2 d. 4 to 5 e. All of these are correct.

14. Where would you find a piece of RNA for certain?

a. 1 b. 3 c. 4 d. 6 e. None of these are correct.

Consider the above replicating fork as the telomeric end and part of a chromosome being replicated till the end.

15. Which end of the two newly synthesized fragments will be shorter than the other.

a. 2 b. 3 c. 4 d. 7 e. None of these are correct.

The table at the right represents a part of your genetic code. Answer following questions that relate to this table.

18. Which of following amino acids is found on a tRNA that has an anticodon of "CCG"?

a. his b. gln c. arg d. gly e. tyr

19. Which of the following has anticodons?

a. mRNA b. tRNA c. rRNA d. proteins e. All of these answers are correct.

20. The only codon for methionine is AUG. Which of the following would be found on the template strand of DNA for this sequence?

a. CAT. b. TAC. c. AUG. d. ATG. e. None of these are correct.

21. The only codon for methionine is AUG. Which of the following would be found on the DNA coding strand for this sequence?
a. CAT. b. TAC. c. AUG. d. ATG. e. None of these are correct.

22. The RNA primer pieces during DNA synthesis are removed by

a. DNA Polymerase I
b. DNA polymerase II
c. DNA polymerase III
d. RNA polymerase
e. None of the above

23. The name of the enzyme capable of synthesizing DNA from RNA template is

a) DNA polymerase
b) RNA polymerase
c) Retrovirus
d) Reverse transcriptase
e) You are a liar. We did not study this.

24. An anticodon sequence is 3'GAI 5'. "I" represents inosine which is a purine. The most likely codon for this is:

a. GAI. b. IAG. c. UUC. d. CUA. e. AUC.