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Mrs Jones, a 60 year old lady who had complained of weakness, headaches, chest pain and weight loss. She had also experienced some memory loss. Mrs Jone's GP had ordered a blood test and it was found that she had megaloblastic (also referred to as macrocytic) anaemia.

1. Describe at least two nutrient deficiencies that could lead to megaloblastic anaemia and discuss the role of these nutrients in red blood cell synthesis and function.

2. Describe what additional tests could be performed to determine the exact cause of the megaloblastic anaemia suffered by Mrs Jone's.

3. Discuss the potential outcomes of the additional tests in relation to your final diagnosis and possible treatments.

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