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Metaphase

This  is a period  of short duration, the  chromosomes are  at the  stage of their  maximum  condensation and , hence more  clearly  visible ,Upon  each  side  of their  centromeres,  a plate or cup like  dise ,formed  of protein  complexes  and  called  kinetochore , appears in the  depression  of the chromatid  of this  side  the  kinetochore  of each side  is seen to be attached  to  a bundle  of a number ( usually  20  to 40 )  of newly  formed spindle  fibres   called discontinuous , or  attachment , or chromosomal ,  or interzonal  spindle  fibres . The  opposite  ends of  these  fibres   are attached to the  spindle poles of their  respective sides. These  fibres pull  the scattered  chromosomes and  align then  in a fixed  specific  manner at the equatorial plane  of the spindle  forming  what is  known  as the equatorial or  metaphase plate.

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