problem 1:

Mention the characteristics and mode of the action of different types of restriction enzymes with ex.

problem 2:

Compare the employ of Lambda and M13 phases with regard to capacity, copy number and ease in manipulations.

problem 3:

Discuss the structure and strategies of the Cosmid Vector.

problem 4:

Discuss the criteria which used to decide if a particular recombinant protein must be produced in yeast, insect or mammalian cell system.

problem 5:

Illustrate the different modes and vehicles for the gene delivery systems adapted in gene therapy for animals.