problem 1) prepare down the types of tissue culture systems used in plant / animal tissue culture. Mention advantages, limitations and applications of any one system.

problem 2) List the landmarks in the area of plant tissue culture. Describe recent development.

problem 3) Describe the bases for selecting specific components for preparing plant tissue culture media. Add a note on importance of pH of nutrient medium.

problem 4) prepare descriptive notes on:

a) Totipotency of plant cells.

b) Methods of separation of cell types.

problem 5) Compare and contrast in vitro organogenesis and embryogenesis in plants.

problem 6) Describe in brief:

a) Secondary metabolism in cultured plant cells.

b) Plant protoplast culture.

problem 8) prepare the commercial applications of animal tissue culture and describe them.

problem 9) prepare explanatory notes on:

a) Mammalian cloning.

b) Genetic manipulation of cultured cells.