Linear double stranded dna- sample lsu1- is digested with 2, Ask an Expert

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Linear double stranded DNA- sample LSU1- is digested with 2 restriction endonucleases (separately and together) and the following bands are observed after electrophoresis ( quantities represent length in bp): BamH1:1,9 and 13.5;EcoR1: 3, 3.5, 4, and 13. BamH1 and EcoR1: 1, 2.5, 3, 4, 5, and 8.

a. How many BamH1 cut sites are present in LSU1 ? Justify your answer in one sentence.

b. How many EcoR1 cut sites are present in LSU1? Justify your answer in one sentence.

c. Based on the given band sizes, construct a restriction endonuclease enzyme site map and illustrate the total size of LSU 1, their reletive position and distance between all adjacent restriction sites.

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Category:- Biology
Reference No.:- M9435603

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