Ask Question, Ask an Expert

+1-415-315-9853

info@mywordsolution.com

Ask Biology Expert

In this experiment, we will change the genotype and therefore the phenotype of a bacterium by genetically transforming an organism by introducing foreign DNA into its genome. This is called transformation.

In this exercise, we will use the bacterial plasmid as a source of transforming DNA. The plasmid we are using is called pUC 118, and it contains a gene that is resistance to the antibiotic ampicillin. Bacteria that contain the plasmid are able to remain alive and grow in the presence of this antibiotic ampicillin; they are called ampicillin resistant bacteria. Bacteria that do not have the plasmid cannot divide and grow in the presence of ampicillin. You will genetically transform ampicillin-sensitive bacteria (without plasmid) into ampicillin-resistant bacteria (with plasmid). The addition of an ampicillin resistance plasmid will change the genotype of the bacterial cell and will therefore change its phenotype. The transformed cells will gain the ability to synthesize an enzyme that destroys ampicillin.

The E. coli bacteria used do not spontaneously take up DNA. Therefore, the E. coli cells need to be pretreated to make them take up DNA more sufficiently. They need to be "competent" cells. Each lab group will mix DNA with the competent E. coli cells. Any cells that takes up the plasmid DNA and maintains it to express the ampicillin-resistance is called a transformant. One half of cells will be plated on ampicillin plates to determine the number of transformants. The other half of cells will be plated on nutrient agar plates as controls. Each student will count the number of transformants obtained.

1. What is genetic transformation?
2. What is a plasmid?
3. What are competent cells?
4. What are the materials needed for genetic transformation experiment?
5. What are the steps in a genetic transformation experiment?
6. When E. coli cells containing the pUC 118 plasmid are placed on growth medium containing 10 times as much ampicillin as we used in our lab, they are unable to grow.
7. describe how a change in genotype results in a change in phenotype?

Biology, Academics

  • Category:- Biology
  • Reference No.:- M928349

Have any Question? 


Related Questions in Biology

Write a paper about pandainstruction is given belowbullyour

Write a paper about PANDA. Instruction is given below. • Your position paper should be from 3 pages in length! (Reference page excluded) • Provide evidence as to why the animal has become endangered and emphasize the eff ...

Explain why nbspin psoriasis is an nbspthe cells in the

Explain why  in Psoriasis is an  the cells in the dermis and epidermis thickened. Decreased cell maturation occurs in psoriasis  as a result of? Reference/s  must be provided to make  sure the  information  you are tutor ...

Hans had had a strep throat two weeks before he was to

Hans had had a strep throat two weeks before he was to start anew job. He had to complete some work before he could resign fromhis old position so he ignored the bacterial infection. In thefirst week of his new job he no ...

What characteristics of sahelanthropus tchadenssis exclude

What characteristics of Sahelanthropus tchadenssis exclude it from being considered human?

Transgenic organismsthe development of transgenic organisms

Transgenic Organisms The development of transgenic organisms is an established technique in many laboratories. A transgenic organism is an organism that includes DNA from another organism in the cells of its body. This D ...

Bacterial srnas activate and repress translation by

Bacterial sRNA's activate and repress translation by exposing or covering RBS (ribosome binding site) on mRNA. Riboswitches also activate and repress translation by exposing or covering RBS on mRNA. How do these two regu ...

Imagine two parents are both heterozygous for two autosomal

Imagine two parents are both heterozygous for two autosomal genes. Assuming independent assortment, what proportion of their offspring are expected to have the same phenotype (for these two traits) as the parents? A) 0.2 ...

1 explain how insulin regulates glucose levels in the

1. Explain how insulin regulates glucose levels in the blood? 2. What is unique about the glands of the endocrine system?

How does complementary base pairing facilitate dna

How does complementary base pairing facilitate DNA replication and the processes of transcription and translation? Identify the base pairs in each process.

What are the ingredients in gatorade how does it provide

What are the ingredients in Gatorade how does it provide energy? In your opinion, should a person use this drink or supplement on a regular basis? Why or why not?

  • 4,153,160 Questions Asked
  • 13,132 Experts
  • 2,558,936 Questions Answered

Ask Experts for help!!

Looking for Assignment Help?

Start excelling in your Courses, Get help with Assignment

Write us your full requirement for evaluation and you will receive response within 20 minutes turnaround time.

Ask Now Help with Problems, Get a Best Answer

Section onea in an atwood machine suppose two objects of

SECTION ONE (a) In an Atwood Machine, suppose two objects of unequal mass are hung vertically over a frictionless

Part 1you work in hr for a company that operates a factory

Part 1: You work in HR for a company that operates a factory manufacturing fiberglass. There are several hundred empl

Details on advanced accounting paperthis paper is intended

DETAILS ON ADVANCED ACCOUNTING PAPER This paper is intended for students to apply the theoretical knowledge around ac

Create a provider database and related reports and queries

Create a provider database and related reports and queries to capture contact information for potential PC component pro

Describe what you learned about the impact of economic

Describe what you learned about the impact of economic, social, and demographic trends affecting the US labor environmen