Ask Question, Ask an Expert

+1-415-315-9853

info@mywordsolution.com

Ask Biology Expert

In this experiment, we will change the genotype and therefore the phenotype of a bacterium by genetically transforming an organism by introducing foreign DNA into its genome. This is called transformation.

In this exercise, we will use the bacterial plasmid as a source of transforming DNA. The plasmid we are using is called pUC 118, and it contains a gene that is resistance to the antibiotic ampicillin. Bacteria that contain the plasmid are able to remain alive and grow in the presence of this antibiotic ampicillin; they are called ampicillin resistant bacteria. Bacteria that do not have the plasmid cannot divide and grow in the presence of ampicillin. You will genetically transform ampicillin-sensitive bacteria (without plasmid) into ampicillin-resistant bacteria (with plasmid). The addition of an ampicillin resistance plasmid will change the genotype of the bacterial cell and will therefore change its phenotype. The transformed cells will gain the ability to synthesize an enzyme that destroys ampicillin.

The E. coli bacteria used do not spontaneously take up DNA. Therefore, the E. coli cells need to be pretreated to make them take up DNA more sufficiently. They need to be "competent" cells. Each lab group will mix DNA with the competent E. coli cells. Any cells that takes up the plasmid DNA and maintains it to express the ampicillin-resistance is called a transformant. One half of cells will be plated on ampicillin plates to determine the number of transformants. The other half of cells will be plated on nutrient agar plates as controls. Each student will count the number of transformants obtained.

1. What is genetic transformation?
2. What is a plasmid?
3. What are competent cells?
4. What are the materials needed for genetic transformation experiment?
5. What are the steps in a genetic transformation experiment?
6. When E. coli cells containing the pUC 118 plasmid are placed on growth medium containing 10 times as much ampicillin as we used in our lab, they are unable to grow.
7. describe how a change in genotype results in a change in phenotype?

Biology, Academics

  • Category:- Biology
  • Reference No.:- M928349

Have any Question? 


Related Questions in Biology

Mrs smith is 50 years of age and is nervous about going

Mrs. Smith is 50 years of age and is nervous about going through menopause. How would you explain the process to her? What effects do estrogen and progesterone have on the vaginal mucosa? How does the effect of estrogen ...

Imagine that you had 11000 of the worlds gross domestic

Imagine that you had 1/1000 of the worlds gross domestic product available at your disposal for conservation biology product? Is it a sound conservation decision to protect just 4% of the earth's living habitat? Why or w ...

Gene technologygene technology carries with it social and

Gene Technology Gene technology carries with it social and ethical implications-many of which engender personal views and discussion. Select one (1) of the following biotechnology topics to write about:  Genetically modi ...

John has difficulty swallowing loss of taste sensation in

John has difficulty swallowing, loss of taste sensation in the posterior one-third of the right half of his tongue, and decreased salivation, especially on the right side. Describe the sensory portion of the affected neu ...

There was no correlation between early death of a male

There was no correlation between early death of a male child and eating habits of his paternal grandmother, but there was a strong correlation with the eating habits of his paternal grandfather. What does this tell you a ...

What effect did grounding of the planes after 911 have on

What effect did grounding of the planes after 9/11 have on daily temperature ranges? Why was temperature affected?

Chronic diseasesresearch paper on diabeteseach student will

CHRONIC DISEASES Research Paper on Diabetes Each student will write a 5-7 page research paper on a chronic disease or an emerging/re-emerging disease using at least 5 or more current sources, APA format, double-spaced, a ...

Topic systemic pathophysiology of the respiratory and

Topic: Systemic Pathophysiology of the Respiratory and Gastrointestinal Systems Objective: Discuss a disease, condition or syndrome affecting the cardiovascular system and current research, events, or interesting facts a ...

Part aprepare a powerpoint slides meeting below grading

PART A Prepare a powerpoint slides meeting below grading criteria covering topic Isoflavones and Polymethoxyflavones using recent original articles one from cell, frontier and nature. -recent articles related to the mech ...

Please solve the problems with correct answers instead of

PLEASE SOLVE THE PROBLEMS WITH "correct" answers, instead of just writing answers. (1) Halophytes are plants that live in moist soils with high salt concentrations. Despite being surrounded my water, halophytes are often ...

  • 4,153,160 Questions Asked
  • 13,132 Experts
  • 2,558,936 Questions Answered

Ask Experts for help!!

Looking for Assignment Help?

Start excelling in your Courses, Get help with Assignment

Write us your full requirement for evaluation and you will receive response within 20 minutes turnaround time.

Ask Now Help with Problems, Get a Best Answer

Section onea in an atwood machine suppose two objects of

SECTION ONE (a) In an Atwood Machine, suppose two objects of unequal mass are hung vertically over a frictionless

Part 1you work in hr for a company that operates a factory

Part 1: You work in HR for a company that operates a factory manufacturing fiberglass. There are several hundred empl

Details on advanced accounting paperthis paper is intended

DETAILS ON ADVANCED ACCOUNTING PAPER This paper is intended for students to apply the theoretical knowledge around ac

Create a provider database and related reports and queries

Create a provider database and related reports and queries to capture contact information for potential PC component pro

Describe what you learned about the impact of economic

Describe what you learned about the impact of economic, social, and demographic trends affecting the US labor environmen