In the shotgun approach to whole-genome sequencing random, Ask an Expert

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In the shotgun approach to whole-genome sequencing (shotgun sequencing), random DNA fragments of a chromosome are sequenced. The fragment sequences are then assembled into a continuous sequence that represents the DNA of the entire chromosome.
What are the steps in the shotgun approach to whole-genome sequencing?
PLACE IN ORDER
A. 1-kb fragments are cloned inot plasminds.
B. Chromosomes copies are broken into 1-kb fragments
C. The plasmids are sequenced.
D. Multiple copies of the smae chromosome are prepared.
E. A computer combines the fragments sequences.
F. 1-kb fragments are transcribed into RNA.

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