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In a generalized transduction experiment, phages are collected from an E. coli donor strain of genotype cys+ leu+ thr+ and used to transduce a recipient of genotype cys- leu- thr-. Initially the treated recipient populations placed on a minimsl medium with leucine and threonine. Many colonies are obtained.

a) What are possible genotypes of these colonies?
b) Strains from theses colonies are then inoculated onto 3 different media. (1) minimal plus threonine only. (2) minimal plus leucine only, and (3) minimial. What genotypes could in theory grow on these 3 media?
c) It is observed that 56% of the original colonoes grow on (1), 5% grow on (2) and no colonies grow on (3). What are the actual genotypes of the colonies on (1),(2) and (3)?
d) Draw a map showing the order of the 3 genes and which of the 2 outer genes is closer to the middle.

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Category:- Biology
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