1) If p-nitro phenyl phosphate had been employed as a substrate, would the same Km have been obtained? Describe.
2) Elevated blood plasma levels of alkaline phosphatase (hyperphosphatasaemia) are an indication of a number of conditions. In brief comment on the use of alkaline phosphatase assays as a diagnostic help for TWO named situations. As well describe the requirement and nature of "follow-up or confirmatory tests.