Identify if each of the following statements about transcription is True or False. Choose true or false. Give explanation.

 True False 

The same strand of a double helix serves as the template for transcription of every gene on the chromosome.

 True False 

The coding strand is complementary to the RNA transcript.

 True False 

The coding strand is similar to the RNA transcript.

 True False 

DNA strands are identical if reading from the 5' end of each complementary strand.

Which one of the following statements about transcription is correct? Give explanation.

 The RNA polymerase from E. coli is able to transcribe tRNA from a eukaryotic DNA template. 
 Eukaryotic transcription factors have no effect on the efficiency of transcription in E. coli. 
 The sigma factor in the eukaryotic RNA polymerase I recognizes -90 and -34 regions of E. coli DNA. 
 The sigma factor in the eukaryotic RNA polymerase I recognizes -90 and -34 regions of eukaryotic DNA. 
 Eukaryotic RNA polymerase I is able to transcribe rRNA from an E. coli DNA template. 

Which one of the following statements about mRNA transcription and processing in eukaryotes isincorrect?

 Mature mRNAs are exported out of the nucleus into the cytoplasm for translation. 
 A single primary transcript can be turned into one of several possible mature mRNAs. 
 A methylguanosine cap is added to the 5' end of each transcript. 
 All splicing occurs in the nucleus. 
 Prior to poly-A tail addition, the 3' end of the transcript is hydrolyzed by an exonuclease. 

Identify if each of the following statements is True or False.

 True False  Eukaryotic mRNAs are usually longer in length than the DNA sequence they are transcribed from. 
 True False  Processing of mRNA in eukaryotes occurs in the nucleus. 
 True False  Removal of introns is an example of post-transcriptional modification. 
 True False  Bacteria use a complex of four snRNPs to remove introns from their primary 

What would be the predicted result of a strain of bacteria which lacks splicing machinery?

 Bacteria do not have splicing machinery or introns, so the bacterial cells will suffer no ill effects. 
 Incorrect proteins will be synthesized since ribosomes cannot recognize introns vs. exons. 
 Cells will be fine since ribosomes recognize introns and will not translate any non-coding RNA. 
 Since introns are not removed, the primary transcript will be translated and proteins will be too large and defective. 
 Less protein will be synthesized since alternative splicing will not occur. 

Which one of the following statements about RNA processing is correct?

 Introns are present in the mature mRNA. 
 In alternative splicing, different combinations of introns are used. 
 Splicing occurs in the nucleus, and cap and tail structure addition occurs in the cytoplasm. 
 The result of alternative splicing is different pre-mRNAs from the same gene. 
 The result of alternative splicing is different mature mRNAs from the same gene.