I use an affinity column to purify a protein x from cell, Ask an Expert

Academics

I use an affinity column to purify a protein, "X" from cell lysate. The specific activity of the protein is very low after this step. So I run the affinity-purified sample through an anion exchanger, and collect the early fraction. This eluate has very high specific activity. My results from a PAGE and Western blot after each step are shown. Also shown are the enzyme kinetics after the affinity purification, and after the anion exchange column. Based on all these data, what is your BEST explanation?

View complete question

Biology, Academics

Category:- Biology
Reference No.:- M91670457

Have any Question?Write your Review or question?

Related Questions in Biology

Bioinformatics assignment -in this assignment should check

Bioinformatics Assignment - In this assignment should check the following sequence and test whether it has the following restriction cut sites. This searching should be done globally, that is, it should check for all pos ...

One page highlighting the current state of federal

One page highlighting the current state of federal legislation for the use of stem cells

Describe at least one significant structural difference

Describe at least one significant structural difference between the human chromosomes and Drosophila Melanogaster chromosomes

If someone has been sitting without standing for 6 hours

If someone has been sitting without standing for 6 hours, and blood has been pooling in their veins, why would they feel dizzy when they stood up? How does this related to stroke volume and mean arterial pressure?

Assignment on nutrition - q1 task you need to select 2

Assignment on Nutrition - Q1. Task: You need to select 2 different age groups of your choice. You will need to plan balanced meals with snacks for a day. Once you have laid out the meal plan you need to: Explain why the ...

Question dietary recommendation and analysis projectin this

Question: Dietary recommendation and analysis project In this project, you will use software to analyze our own diet and make dietary recommendation. You will need submit a report. You can choose any of software if you f ...

Diffusion and osmosis questionin your large intestine the

Diffusion and osmosis question In your large intestine, the water from the food you have eaten needs to be kept in the body to prevent dehydration. Therefore the high concentration of water in feces needs to be moved int ...

Assignment 1 create at least a 350-word blog post in

Assignment 1: Create at least a 350-word blog post in Microsoft® Word in response to the following question: Female copperhead snakes have the ability to reproduce both sexually and asexually. In your opinion, which meth ...

Question this is the final project of the case

Question: This is the final project of the case study 1. Identify the specific case study you chose and the tool(s) you would employ to analyze the healthcare environment. 2. Provide an explanation of how the selected to ...

Why chemically is nitrogen not more available to living

Why, chemically, is nitrogen not more available to living things? I am looking for the simple chemical reason here

4,153,160 Questions Asked
13,132 Experts
2,558,936 Questions Answered

Ask Experts for help!!

Looking for Assignment Help?

Start excelling in your Courses, Get help with Assignment

Write us your full requirement for evaluation and you will receive response within 20 minutes turnaround time.

Ask Now Help with Problems, Get a Best Answer

Recent Questions

Ask Biology Expert