Problem: You just got hired to work for a biotech company that manufactures human insulin protein for diabetic treatment and your job is to express and purify lots of insulin protein using E. coli bacteria. Explain: what type of DNA you would use insert into your plasmid, how you would generate this DNA, how you would get the DNA into the plasmid and how the protein would be expressed and isolated from the bacterial cells? Provide justifications and citations for your responses.