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How would DNA replication be affected if ligase were not available?

A: The template strands would not be able to separate.

B: Replication would result in many small segments of DNA instead of a complete molecule.

C: Complementary RNA would be produced but not complementary DNA.

D: The DNA strands would separate but replication would not be able to start.

E: The DNA strands produced by replication would not be complementary to the template strands.

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