Ask Question, Ask an Expert


Ask Biology Expert

1. You want to infect 10 plates of cells with virus "X" at an MOI of 20. You use a volume of 0.5 ml for the infections. You have 2 x 106 cells on each of the dishes you want to infect, and your virus "X" stock contains 1.25 x 109 PFU/ml. Describe a dilution scheme that will give you the appropriate amount and concentration of virus needed for your infection.

2. How much 4X sample buffer do you need to add to 10 ul of DNA in order to run it out on an agarose gel?

3. A 100 mm dish has a surface area of 57 cm2, and a 60 mm dish has a surface area of 19 cm2. How many 60 mm dishes can you make from five 100 mm dishes if you passage the cells at a 1:5 ratio?

4. You need to stain cells with neutral red prepared in Hank's buffered saline. Neutral red is prepared as a 40X stock in distilled water. How much of this stock solution do you need in order to prepare 200 ml of the stain?

5. Some solutions are prepared as percent weight/weight (% w/w). For ex, a 50% (w/w) sucrose solution is prepared by adding 50 grams of sucrose and 50 ml (50 g) of water. Can you apply the same formulas used in this class to dilute the sucrose solution? Why or why not?

6. You need 200 ml of physiological saline (0.9% NaCl). At your disposal is a 1M (58.44 g/L) stock of NaCl. What volume of the stock solution and how much water should you use to make the 0.9% solution?

What is the molarity of salt in physiological saline?

Biology, Academics

  • Category:- Biology
  • Reference No.:- M944445

Have any Question? 

Related Questions in Biology

Compare the values of q and keq is the physiological

Compare the values of Q and K'eq. Is the physiological reaction near or far from equilibrium? Explain. What does this experiment suggest about the role of PFK-1 as a regulatory enzyme?

Microscopy for microbiology - use and functionhands-on labs

Microscopy for Microbiology - Use and Function Hands-On Labs, Inc. Version 42-0249-00-02 Lab Report Assistant This document is not meant to be a substitute for a formal laboratory report. The Lab Report Assistant is simp ...

Part i textbook questionschapter 38 describe brieflyathe

Part I. Textbook Questions Chapter 3: 8. Describe briefly: a. The genotype of a person who has sickle-cell anemia. b. The genotype of a person with a normal phenotype who has a child with sickle-cell anemia. c. The total ...

What type of solution is 10 dextrose to the

What type of solution is 10% dextrose to the cell?  Assuming dextrose is a non penetrating solute, in which direction will net diffusion occur (into the cell; out of the cell; no movement?

One important aspect of understanding health care in the us

One important aspect of understanding health care in the U.S. involves an analysis of the "Key Indexes of Health and Disease," as they are termed. Review the material on this topic in your textbook and other sources, and ...

Biology1is biology becoming more interesting to you as you

Biology 1. Is biology becoming more interesting to you as you learn about the workings of the cell and how cells interact in an organism? 2. What scientific information have you learned about cells surprises or horrifies ...

Introduction of disease provide a brief description of the

Introduction of disease. Provide a brief description of the disease/disorder. This part of the paper should not be limited to the definition of the disease.Include epidemiology as appropriate.Etiology Identify common cau ...

Questionkayla brings riley now 18 months in for complaint

Question: Kayla brings Riley, now 18 months in for complaint of fever and cough. The fever began 3 days ago and is of normal levels in the morning then gets as high as 100 to 101 degrees in the afternoons. Riley has also ...

Ethical decisions in the workplaceafter reading your

Ethical Decisions in the Workplace After reading your background readings, please review the following scenario and address the questions at the end. Jerry Stevens heads up an excavation crew for the municipal utilities ...

Genetics and inheritancereview information on genetic

Genetics and Inheritance Review information on genetic disorders on the National Human Genome Research Institute Site: Choose a genetic disorder that can be inherited from a parent. Write a 525- to ...

  • 4,153,160 Questions Asked
  • 13,132 Experts
  • 2,558,936 Questions Answered

Ask Experts for help!!

Looking for Assignment Help?

Start excelling in your Courses, Get help with Assignment

Write us your full requirement for evaluation and you will receive response within 20 minutes turnaround time.

Ask Now Help with Problems, Get a Best Answer

A cola-dispensing machine is set to dispense 9 ounces of

A cola-dispensing machine is set to dispense 9 ounces of cola per cup, with a standard deviation of 1.0 ounce. The manuf

What is marketingbullwhat is marketing think back to your

What is Marketing? • "What is marketing"? Think back to your impressions before you started this class versus how you

Question -your client david smith runs a small it

QUESTION - Your client, David Smith runs a small IT consulting business specialising in computer software and techno

Inspection of a random sample of 22 aircraft showed that 15

Inspection of a random sample of 22 aircraft showed that 15 needed repairs to fix a wiring problem that might compromise

Effective hrmquestionhow can an effective hrm system help

Effective HRM Question How can an effective HRM system help facilitate the achievement of an organization's strate