Cystic fibrosis is an autosomal recessive disease in which the cystic fibrosis transmembrane conductance regulator (CFTR) protein is abnormal. The transcribed portion of the cystic fibrosis gene spans about 250,000 base pairs of DNA. The CFTR protein, with 1,480 amino acids, is translated from an mRNA of about 6,500 bases. The most common mutation in this gene results in a protein that is missing a phenylalanine at position 508 (?F508).

1. How many nucleotides are translated in the CFTR protein?

2. With regard to the ?F508 mutation: At the DNA level, what specific alteration has occurred? (3 points)

3. Show the exact change in sequence of #2