How many Nuclear Pores are there per nucleus? How is that of any relevance?

Why is it that the NPC restricts the passage of proteins across its lumen?

How is the RanGTP gradient across the Nuclear Envelope generated? Where is the Ran GTPase Activating Protein (RanGAP) localized? Where is the Ran GTP Exchange Factor localized? What does each one of these factors do?

How are proteins imported into the nucleus? Which are the factors required? How are proteins exported out of the nucleus? Which are the factors required?

How is nuclear import/export regulated?

How do you figure out the location of NLSs/NESs in proteins that are shuttled across the NPC? (Remember to check the in-class activity related to mapping NLSs/NESs in proteins).

How are chromosomes distributed within the nucleus?

What are Replication Factories, Nuclear Speckles, Pro-Myelocytic Leukemia Nuclear Bodies (PML-NBs), and Nucleoli (Nucleolus)?

What's the critical event that triggers nuclear disassembly? What's the post-translational modification that regulates it?

How is the nucleus reassembled after mitosis? What are the key molecular interactions that drive its reassembly?