A) How is the gene for β-galactosidase used in the construction of a plasmid?
The gene is a promoter that is sensitive to the presence of the sugar, galactose.
It is an origin of replication.
It is a cloning site.
It is a marker for insertion of DNA.
B) A DNA library is
an orderly array of all the genes within an organism
a collection of vectors
the collection of plasmids found within a single E. coli
a collection of DNA fragments representing the entire genome of an organism
C) What enzyme is needed in order to make a cDNA library?
DNA helicase
RNA polymerase
Reverse transcriptase
Restriction exonuclease