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How is plasmid DNA purified from RNA and genomic DNA?
-What are reasons behind using the following chemicals in the genomic DNA isolation: Liquid nitrogen, Phenol/chloroform, Isopropanol, RNase
-How will you make a 0.8% agarose gel (250 ml volume) in 1x TAE with a 50X TAE? Show calculations and describe the procedure. 

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