The following experimental data were collected during a study of the catalytic activity of an intestinal peptidase with the substrate glycylglycine:
Glycylglycine + H2O ï?® 2 glycine
[S] (mM) Product formed (?mol/min)
1.5 0.21
2.0 0.24
3.0 0.28
4.0 0.33
8.0 0.40
16.0 0.45
Graph the above data to determine the Km and Vmax for this enzyme preparation and substrate.