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Hexokinase can use glucose or fructose as a substrate. The Km forglucose is 0.15 mM and for fructose Km = 1.5 mM. Assume vmax forboth substrates.
a. For which substrate does hexokinase have the highest affinity?Why?
b. Calculate V as a percentage of Vmax for each substate when [S]equals 0.15mM.

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