Gtp is again added to the redissolved pellet proteins and, Ask an Expert

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Q. A standard procedure in purifying tubulin is to extract tissue on ice, after that add GTP and warm the tissue extract to 37°C. The warmed extract is next centrifuged at a speed insufficient to pellet average sized proteins, but enough to pellet large protein complexes. After centrifugation, the supernatant is discarded, and the pellet is redissolved in cold buffer. GTP is again added to the redissolved pellet proteins, and the solution is again warmed for a few minutes. Then centrifugation at similar speed described above is again performed. This process is repeated several times.

How will this procedure result in purification of tubulin?

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