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Glycolysis is believed to be one of the most ancient of metabolic processes. Which statement below least supports this idea?

1. If run in reverse, glycolysis will build glucose molecules.

2. Glycolysis neither uses nor needs O2.

3. Glycolysis is found in all eukaryotic cells.

4. The enzymes of glycolysis are found in the cytoplasm rather than in a membrane-enclosed organelle.

5. Bacteria, the most primitive cells, make extensive use of glycolysis.

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