problem 1: Give an account on restriction endonucleases and their categorization.

problem 2: prepare about the artificial chromosomes BAC and YAC.

problem 3: Give an account on the Gene transfer methods used in genetic engineering.

problem 4: Describe basic principle and variations of PCR.

problem 5: Give an account on the human genome project (HGP).

problem 6: Describe the significance of DNA ligase and reverse transcriptase.

problem 7: Give an account on the viral vectors.

problem 8: What do you mean by the term reported gene assay? Describe with illustrations.

problem 9: Describe the significance of southern and northern blotting methods.

problem 10: Give an account on the applications of genetic engineering in agriculture.